From owner-biotechniques@net.bio.net Wed Jan 03 22:00:00 1996 Path: biosci!biosci!not-for-mail From: cmody@acs.ucalgary.ca Newsgroups: bionet.journals.letters.biotechniques Subject: Spectrophotometric lipid concentration assay Date: 4 Jan 1996 07:02:51 -0800 Organization: The University of Calgary Lines: 10 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Our objective is to determine the lipid concentration in yeast (cryptococcal) cell fractions acquired from glass bead mill homogenization. Concentrations of DNA, protein, and carbohydrate have already been determined spectrophotometrically. In order to complete the characterization of the aforementioned fractions lipid concentrations must be assayed. Any replies with recommendations of an inexpensive, non-radioactive lipid assay would be much appreciated. Thank you for your time. tfbruno@acs.ucalgary.ca .