From owner-celegans@net.bio.net Wed Sep 01 12:38:00 1999 Path: biosci!biosci!not-for-mail From: cn0@mole.bio.cam.ac.uk (Cara Neades) Newsgroups: bionet.celegans Subject: M9 buffer Date: 1 Sep 1999 06:37:53 -0700 Organization: MRC Human Genome Mapping Project Resource Centre Lines: 21 Sender: daemon@net.bio.net Approved: wmorgan@acs.wooster.edu Distribution: world Message-ID: <7qja7h$pd1@net.bio.net> NNTP-Posting-Host: net.bio.net Hello, I'm trying to make M9 buffer, but there is a fine particulate after autoclaving. Has anybody had this problem before? I suspect the MgSO4 is the culprit, would adding it after autoclaving help? Thanks! ----------- Cara Neades Wellcome/CRC Institute Tennis Court Road Cambridge CB2 1QR United Kingdom +44 (0)1223 334144 --- .