From owner-diagnost@hgmp.mrc.ac.uk Tue Aug 20 10:21:56 2002 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id D9D0717BFB for ; Tue, 20 Aug 2002 10:21:53 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 1707717BE8 for ; Tue, 20 Aug 2002 10:21:48 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6045) id E3EED17C00; Tue, 20 Aug 2002 10:19:49 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 3B63617D36 for ; Tue, 6 Aug 2002 18:36:51 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id EBB4217C53; Tue, 6 Aug 2002 18:36:45 +0100 (BST) To: bionet-diagnostics@net.bio.net From: Alan.Wheeler@gov.ky ("Wheeler, Alan") Newsgroups: bionet.diagnostics Subject: Question about agarose gel electrophoresis Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <4F3534137955D3119C2400508B0EC60A07353FEE@SVREXCH> MIME-Version: 1.0 Content-Type: multipart/alternative; boundary="----_=_NextPart_001_01C23D6F.C6675480" X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 660D317D37 for ; Tue, 6 Aug 2002 18:36:43 +0100 (BST) X-Received: from svrexch.exchange.gov.ky (unknown [209.27.55.193]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id C6F5117C53 for ; Tue, 6 Aug 2002 18:36:34 +0100 (BST) X-Received: by SVREXCH with Internet Mail Service (5.5.2653.19) id ; Tue, 6 Aug 2002 12:36:26 -0500 X-To: "'diagnost@net.bio.net'" X-Mailer: Internet Mail Service (5.5.2653.19) Date: Tue, 20 Aug 2002 10:19:49 +0100 (BST) X-Razor-id: 6470471b91e884822915aacf119d5da58a04788d Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk This message is in MIME format. Since your mail reader does not understand this format, some or all of this message may not be legible. ------_=_NextPart_001_01C23D6F.C6675480 Content-Type: text/plain; charset="iso-8859-1" I am just starting to monitor for West Nile Virus using PCR. I expect to be getting a large number of negative samples. I am wondering if it is possible to re-use a gel if all the samples run in it come up negative. What I was thinking of doing would be to prepare 24 samples and run them three times in the same gel. Please send any replies directly to me because I have not yet signed up to this group. regards Alan Disclaimer The contents of this e-mail are confidential to the addressee and are intended solely for their exclusive use. If you are not the addressee, you have received this e-mail in error and any disclosure, copying, distribution or action taken in reliance on it is prohibited and may be unlawful. Any opinions expressed in this e-mail are those of the author and do not necessarily reflect those of the Cayman Islands Government or anyone associated thereof. Visit the official website of the Cayman Islands Government at http://www.gov.ky ------_=_NextPart_001_01C23D6F.C6675480 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Question about agarose gel electrophoresis

I am just starting to monitor for West = Nile Virus using PCR. I expect to be getting a large number of negative = samples. I am wondering if it is possible to re-use a gel if all the = samples run in it come up negative. What I was thinking of doing would = be to prepare 24 samples and run them three times in the same gel. =

Please send any replies directly to me = because I have not yet signed up to this group.

regards

Alan

Disclaimer

The contents of this e-mail are = confidential to the addressee and are intended solely for their = exclusive use. If you are not the addressee, you have received this = e-mail in error and any disclosure, copying, distribution or action = taken in reliance on it is prohibited and may be unlawful.

Any opinions expressed in this e-mail = are those of the author and do not necessarily reflect those of the = Cayman Islands Government or anyone associated thereof.

Visit the official website of the = Cayman Islands Government at http://www.gov.ky=20

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