From owner-biotechniques@net.bio.net Mon Mar 09 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Mary McCarthy Newsgroups: bionet.journals.letters.biotechniques Subject: BioTechniques 24(3), March 1998 Date: 10 Mar 1998 08:39:21 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 143 Sender: daemon@net.bio.net Approved: mmccarthy@BioTechniques.com Distribution: world Message-ID: <6e3qbp$5pt@net.bio.net> NNTP-Posting-Host: net.bio.net BioTechniques 24(3), March 1998 Tale of Contents Benchmarks Low-Voltage Separation of Phosphoamino Acids by Silica Gel=20 Thin-Layer Electrophoresis in a DNA Electrophoresis Cell S.C. Hardin and S.M. Wolniak In Situ Detection Method for Glutaminyl Cyclase Activity in Polyacrylamide Gels J.B. Houseknecht, J.S. Temple and R.C. Bateman, Jr. Analysis of Cell-Cycle Profiles in Transfected Cells Using a=20 Membrane-Targeted GFP W. Jiang and T. Hunter Chemiluminescence-Based Detection of Minute Amounts of=20 Apoptotic DNA F.Lopez Blanco, J. Gonzalez-Reyes, L.F. Fanjul, C.M. Ruiz de Galarreta and J. Quintana Aguiar Rapid Screening of Fusion Protein Recombinants by Measuring=20 Effects of Protein Overexpression on Cell Growth G.D. Davis and R.G. Harrison Versatile Low-Copy-Number Plasmids for Temperature-Inducible=20 Overexpression of Bacterial Genes in Escherichia coli C. Gotting, G. Thierbach, A. Puhler and J. Kalinowski =20 Chemiluminescence-Based RNase Protection Assays for Simultaneous Quantification of Procollagen mRNAs Containing AU-Rich Regions M.J. Ausserlechner, R. Sgonc and G. Wick Modification of Primer Design Facilitates the Use of Differential Display M.S. Brenz Verca, S. Brenz Verca, S. Rusconi and J.-L. Dreyer Use of Novel Downstream Primers for Differential Display RT-PCR X. Wang, X. Li and G.Z. Feuerstein Rapid Cloning of Telomere-Associated Sequence Using Primer- Tagged Amplification G. Fu and D.C. Barker PCR-Assisted cDNA Cloning: A Guided Tour of the Minefield R. Hooft van Huijsduijnen Use of the Restriction Enzyme AvaI and Exo- Bst Polymerase in Strand Displacement Amplification M.A. Milla, P.A. Spears, R.E. Pearson and G.T. Walker =20 Long-Distance Genome Walking Using the Long and Accurate=20 Polymerase Chain Reaction G.-S. Min and J.R. Powell Relative Amplification Efficiency of Differently Sized Templates by Long-Distance PCR G. Sanchez, D. Gautheret, X. Xu, A.-L. Chenine and I. Hirsch Re-amplification of Short Primer-Generated Bands from RAPD and Methylation-Sensitive Restriction Fingerprinting by Discrimination Primers M. Zeng, E.O. Martsen and J.-N. Lapeyre Direct Amplification of Microsatellite Alleles from Sonicated=20 Goldfish Sperm W. Zheng, N. Stacey, D. Liu and C. Strobeck Construction of a Device Composed of Common Plumbing Supplies=20 for Freezing Microscopy Samples D.W. Darnowski and L.O. Vodkin Simultaneous Purification of RNA and DNA from Liver Using=20 Sodium Acetate Precipitation J.K. Evans, P. Troilo and B.J. Ledwith Internet On-Ramp Cyberspace for Biologists Short Technical Reports Random Mutagenesis by Whole-Plasmid PCR Amplification A. Parikh and F.P. Guengerich Long PCRs of Transposons in the Structural Analysis of Genes=20 Encoding Acquired Glycopeptide Resistance in Enterococci H. Haaheim, K.H. Dahl, G.S. Simonsen, O. Olsvik and A. Sundsfjord Bacterial Growth Medium that Significantly Increases the Yield of Recombinant Plasmid H.M. Duttweiler and D.S. Gross 5=A2-Degenerate 3=A2-Dideoxy-Terminated Competitors of PCR Primers Increase Specificity of Amplification S.P. Atamas, I.G. Luzina, B.S. Handwerger and B. White=20 Small-Scale Isolation of Genomic DNA from Streptomyces Mycelia=20 or Spores A.J. Kutchma, M.A. Roberts, D.B. Knaebel and D.L. Crawford =20 Simultaneous Detection of Two GFP Spectral Mutants During In Vivo Confocal Microscopy of Migrating Dictyostelium Cells T. Zimmerman and F. Siegert Research Reports Development and Applications of Enhanced Green Fluorescent=20 Protein Mutants R.H. Stauber, K. Horie, P. Carney, E.A. Hudson, N.I. Tarasova, G.A. Gaitanaris and G.N. Pavlakis Localization of Trinucleotide Repeat Sequences in Myotonic Dystrophy Cells Using a Single Fluorochrome-Labeled PNA Probe K.L. Taneja Product Application Focus Fusion of Green Fluorescent Protein with the Zeocin=D4-Resistance=20 Marker Allows Visual Screening and Drug Selection of Transfected Eukaryotic Cells R.P. Bennett, C.A. Cox and J.P. Hoeffler FastTag Nucleic Acid Labeling System: A Versatile Method for Incorporating Haptens, Fluorochromes and Affinity Ligands into=20 DNA, RNA and Oligonucleotides S.G. Daniel, M.E. Westling, M.S. Moss and B.D. Kanagy Ad Hoc Reviewers Literature Express New Products Index to Advertisers .