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Subject: BioTechniques 23(1), July 1997
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BioTechniques 23(1), July 1997
Table of Contents

BENCHMARKS

Rapid quantification of DNA samples extracted
from buccal scrapes prior to DNA profiling
     Hopwood, A., Oldroyd, N., Fellows, S.,
     Ward, R., Owen, S.-A., and Sullivan, K.

Construction of a library for sequencing long
regions of malarial genomic DNA
     Werner, E., Patel, K., and Holder, A.A.

Reversal of RT-PCR inhibition observed in
heparinized clinical specimens
     Jung, R., Lubcke, C., Wagener, C., and
     Neumaier, M.

mRNA differential display in agarose gels
     Rompf, R. and Kahl, G.

Quantitative RT-PCR using a PCR-generated
competitive internal standard
     Fille, M., Shanley, J.D., and
     Aslanzadeh, J.

Combining multiplex and touchdown PCR to
screen murine microsatellite polymorphisms
     Rithidech, K.N., Dunn, J.J., and Gordon,
     C.R.

Minisatellite variant repeat
coding using a semiautomatic system
     Bellas, S., Soto, J.L., Carracedo, A.,
     and Rodriguez-Calvo, M.S.

Efficient non-PCR-mediated overlap extension
of PCR fragments by exonuclease "end
polishing"
     Jespersen, T., Duch, M., and Pedersen,
     F.S.

Combination of direct DNA sequencing with
degenerate primer-mediated PCR and
5'-/3'-RACE to screen novel cDNA sequences
     Fang, C., Mkrtchian, S., and
     Ingelman-Sundberg, M.

Sequence-dependent separation of
DNA fragments by capillary electrophoresis in
the presence of SYBR Green I
     Takai, H., Yamakawa, H., Ohara, O., and
     Sakaguchi-Inoue, J.


Determination of molecular weights of nucleic
acid-binding proteins by UV
photo-crosslinking and SDS-PAGE
     Bourbonniere, M., Shekarabi, M., and
     Nalbantoglu, J.

EGFP-containing vector system that
facilitates stable and transient expression
assays
     Alexander, L., Lee, H., Rosenzweig, M.,
     Jung, J.U., and Desrosiers, R.C.

Reliable method of isolating transfected
clones from the LNCaP human prostatic cell
line
     Bennett, S., Joshua, A., and Russell,
     P.J.

Rapid microplate assay for substrates and
inhibitors of proteinase mixtures
     Oppert, B., Kramer, K.J., and McGaughey,
     W.H.

Isolation of site-specific insert probes from
chimeric YACs
     Petek, E., Kroisel, P.M., and Wagner, K.

Method of data analysis that elucidates
contributions to the T-cell receptor
repertoire
     Daniel, E.S., Gadag, V.G., and Haegert,
     D.G.

Intracellular expression and purification of
the c-kit receptor kinase domain in Pichia
pastoris
     Lam, L.P.Y. and Berger, S.A.

Generation of a moveable poly(A)+ cassette
     Cascone, P.J. and Schwartz, L.M.

Detection and analysis of living, growth-
inhibited mammalian cells following
transfection
     Zeng, Y.-X., Somasundaram, K., Prabhu,
     N.S., Krishnadasan, R., and El-Deiry,
     W.S.

Epitope tag-antibody combination useful for
the detection of protein expression in
prokaryotic and eukaryotic cells
     Rudiger, M., Jockusch, B.M., and
     Rothkegel, M.

Direct primer walking of P1 plasmid DNA
     Benes, V., Kilger, C., Voss, H., Paabo,
     S., and Ansorge, W.

Fabrication of ion-selective microelectrodes
by a centrifugation/suction method
     Woodruff, R.I.

Magnetic bead technology aids sequence
determinations for the 3'- and 5'-ends of
viral RNAs
     Koenig, R.


THE INTERNET ON-RAMP
     Cyberspace for Biologists


SHORT TECHNICAL REPORTS

Mini-exon epitope tagging for analysis of
the protein coding potential of genomic
sequence
     Smith, D.J.

Direct hybridization of large-insert genomic
clones on high-density gridded cDNA filter
arrays
     Kern, S. and Hampton, G.M.

Cycle sequencing of filamentous phage DNA
using a biotinylated primer and delta Taq DNA
polymerase
     Qiu, J., Zhou, H., Aceto, J.F., and
     Kieber-Emmons, T.

Presence of RNase A causes aberrant DNA band
shifts
     Benore-Parsons, M. and Ayoub, M.A.


RESEARCH REPORTS

Amplification of 4-9-kb human genomic DNA
flanking a known site using a panhandle PCR
variant
     Jones, D.H. and Winistorfer, S.C.

Protein/amphipathic polyamine complexes
enable highly efficient transfection with
minimal toxicity

     Budker, V., Hagstrom, J.E., Lapina, O.,
     Eifrig, D., Fritz, J., and Wolff, J.A.


PRODUCT APPLICATION FOCUS

Fluorescence imaging in human identity
testing
     Worley, J., Lee, S., Ma, M.S.,
     Eisenberg, A., Chen,H.-Y., and
     Mansfield, E.

LITERATURE EXPRESS
NEW PRODUCTS
INDEX TO ADVERTISERS

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