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Subject: BioTechniques 20(6), June 1996
Date: 29 May 1996 05:49:04 -0700
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BioTechniques 20(6), June 1996
Table of Contents

BENCHMARKS

Joining of DNA fragments by repeated cycles of
denaturation, annealing and extension
     -Kim J., M. Puder and R.J. Soberman

Differentiating allele combinations of a
transgene using multiple primer PCR.
     -Pekhletski R. and D.R. Hampson

An in vitro assay of =E1-galactosidase from
yeast
     -Schneider S., M. Buchert and C.M. Hovens

Improved in situ hybridization: color
intensity enhancement procedure for the
alkaline phosphatase/Fast Red system.
     -Chiu K.-p., T. Sullivan and S.        =20
      Bursztajn

Use of UV methods for measurement of protein
and nucleic acid concentrations
     -Manchester K.L.

Reusing the same blood-stained punch for
sequential DNA amplifications and typing
     -Del Rio S.A., M.A. Marino and P. =20
      Belgrader

DNA extraction technique for PCR in Rain
Forest Plant Species
     -Scott K. and J. Playford

Direct PCR-screening of Pichia pastoris
clones
     -Linder S., M. Schliwa and E.          =20
      Kube-Granderath

Trichloroacetic acid precipitation by
ultracentrifugation to concentrate dilute
protein in viscous solution
     -Hwang B.J. and G. Chu

A modified unique site elimination
mutagenesis in constructing a chloramphenicol
resistance-encoding pGEM vector
     -Young C., K. Matsubara and C. Nitz

Bi-directional Dideoxy Fingerprinting (Bi-
ddF): Rapid and Efficient Screening for
Mutations in the Big BlueTM Transgenic Mouse
Mutation Detection System
     -Haavik, J., H. Nishino, Q. Liu and S.S.
      Sommer


THE INTERNET ON-RAMP


SHORT TECHNICAL REPORTS

Modulaton of non-templated nucleotide
addition by Taq DNA polymerase: primer
modifications that facilitate genotyping.
     -Brownstein M.J., J.D. Carpten and J.R.
      Smith

Optimization of DNase I removal of
contaminating DNA from RNA for use in
quantitative RNA-PCR
     -Huang Z., M.J. Fasco and L.S. Kaminsky

Method for 96-well M13 DNA template
preparations for large scale sequencing
     -Andersson B., J. Lu, K.E. Edwards, D.M.
      Muzny and R.A.Gibbs


BIOFEEDBACK


RESEARCH REPORTS

Differential display protocol with selected
primers that preferentially isolates mRNAs of
moderate-to-low abundance in a microscopic
system
     -Ikonomov O.C. and M.H. Jacob

Generation of sequence signatures from DNA
amplification fingerprints with mini-hairpin
and microsatellite primers
     -Caetano-Anoll=82s G. and P.M. Gresshoff

DNA sequencing by capillary electrophoresis
using short oligonucleotide primer libraries
     -Ruiz-Martinez M.C., E. Carrilho, J.   =20
      Berka, J. Kieleczawa, A.W. Miller, F. =20
      Foret, S. Carson and B.L. Karger

Phage display shot-gun cloning of ligand
binding domains of prokaryotic receptors
approaches 100% correct clones
     -Jacobsson K. and L. Frykberg



BIOCOMPUTING

System for accurate one-dimensional gel
analysis including high resolution
quantitative footprinting
     -Smith J. and M. Singh

Spreadsheet macro for setting up PCR assay
tubes
     -Stowe R.P. and D.L. Pierson


PRODUCT APPLICATION FOCUS

Software to determine optimal oligonucleotide
sequences based on hybridization simulation
data
     -Hyndman D., A. Cooper, S. Pruzinsky, D.
      Coad and M.Mitsuhashi

Selection of murine lymphoid and
hematopoietic cells using polystyrene tissue
culture devices containing covalently
immobilized antibody
     -Berson A.E., K.M. Knobel, D. Rood, K. =20
      Chen, D. Lamons, M.A.McNally, T.B.    =20
      Okarma and J.S. Lebkowski

1996 AUTHORS INDEX (Volume 20)
NEW PRODUCTS
INDEX TO ADVERTISERS


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