From owner-dros@hgmp.mrc.ac.uk Fri Mar 1 13:37:47 2002 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 8CCE217A98 for ; Fri, 1 Mar 2002 13:37:45 +0000 (GMT) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 5512317B00 for ; Fri, 1 Mar 2002 13:37:41 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id A9F2B17A98; Fri, 1 Mar 2002 13:37:38 +0000 (GMT) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 10E3917ABA for ; Thu, 28 Feb 2002 17:33:35 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 8FD4717B25; Thu, 28 Feb 2002 17:33:33 +0000 (GMT) To: bionet-drosophila@net.bio.net From: publicterminal@aecom.yu.edu (publicterminal) Newsgroups: bionet.drosophila Subject: Epitope tagging in the eye disc Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <3C7E6807.A1D8704@aecom.yu.edu> MIME-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 194F817ABA for ; Thu, 28 Feb 2002 17:33:31 +0000 (GMT) X-Received: from mailgw.aecom.yu.edu (mailgw.aecom.yu.edu [129.98.1.16]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 4305817AE6 for ; Thu, 28 Feb 2002 17:33:17 +0000 (GMT) X-Received: from mailvx.aecom.yu.edu (mailvx.aecom.yu.edu [129.98.1.17]) by mailgw.aecom.yu.edu (8.11.6/8.11.6) with SMTP id g1SHXGB07076 for ; Thu, 28 Feb 2002 12:33:16 -0500 X-Received: from post.aecom.yu.edu ([129.98.1.4]) by mailvx.aecom.yu.edu (NAVGW 2.5.1.19) with SMTP id M2002022812331628791 for ; Thu, 28 Feb 2002 12:33:16 -0500 X-Received: from aecom.yu.edu (medlpc4.aecom.yu.edu [129.98.45.104]) by post.aecom.yu.edu (8.9.3/8.9.3) with ESMTP id MAA01266 for ; Thu, 28 Feb 2002 12:33:15 -0500 (EST) X-Mailer: Mozilla 4.77 [en] (Win98; U) X-Accept-Language: en X-To: dros@net.bio.net Date: Fri, 1 Mar 2002 13:37:38 +0000 (GMT) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk I am looking into possible epitopes to use for tagging the N terminal of a protein that I plan to over-expressed in the eye disc. Does anyone know which tags have worked well in the eye imaginal disc and which antibodies to them produce minimum cross reaction with other proteins? Thankyou L Firth --- .