From owner-dros@hgmp.mrc.ac.uk Mon Feb 4 15:06:29 2002 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 8D78517AF2 for ; Mon, 4 Feb 2002 15:06:27 +0000 (GMT) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 8C10717A94 for ; Mon, 4 Feb 2002 15:06:22 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id A79EC17A8D; Mon, 4 Feb 2002 15:06:21 +0000 (GMT) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id BD0E017B01 for ; Fri, 1 Feb 2002 13:20:56 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id BDA1B17B0B; Fri, 1 Feb 2002 13:20:55 +0000 (GMT) To: bionet-drosophila@net.bio.net From: Caroline.Grabbe@ucmp.umu.se (Caroline Grabbe) Newsgroups: bionet.drosophila Subject: Phalloidin problems! Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <4.1.20020201145136.00a0c8a0@panther.cmb.umu.se> X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 6D30917B01 for ; Fri, 1 Feb 2002 13:20:52 +0000 (GMT) X-Received: from mail.umu.se (custer.umdac.umu.se [130.239.8.14]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id E3B6717AFA for ; Fri, 1 Feb 2002 13:20:50 +0000 (GMT) X-Received: from ruth (ruth.ucmp.umu.se [130.239.193.226]) by mail.umu.se (8.8.8/8.8.8) with SMTP id OAA06004 for ; Fri, 1 Feb 2002 14:20:47 +0100 (MET) X-Sender: cargra@panther.cmb.umu.se (Unverified) X-Mailer: QUALCOMM Windows Eudora Pro Version 4.1 X-To: dros@net.bio.net Date: Mon, 4 Feb 2002 15:06:21 +0000 (GMT) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Hi everybody! Lately IŽve been trying to stain embryos and discs with phalloidin, but have come across some problems. I now wonder if you have some advise about phalloidin, for example, from where do you get the best phalloidin and what kind of protocol works best for you? IŽd be very grateful if you can give me some advise and help me solve my problems. Thanks very much! /Caroline Caroline Grabbe UCMP Umeć University Building 6L 901 87 Umeć Sweden Tel: 0046 90 785 67 93 --- .