From owner-celegans@net.bio.net Tue Nov 01 22:00:00 1994
Path: biosci!EATWORMS.SWMED.EDU!leon
From: leon@EATWORMS.SWMED.EDU ("Leon Avery")
Newsgroups: bionet.celegans
Subject: SUMMARY: gut fluorescence
Date: 2 Nov 1994 06:01:11 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Cori Bargmann says:

    starved worms have way less gut granules.  way less.
    if you can stand to fix them, fixing utterly destroys gut fluorescence.
    jin figured that out.  just a short paraformaldehyde fix a la antibody
    staining (an hour or so).

Thomas Burglin says:

    supposedly the flu mutants out there are of no use for such
    purposes.
    The vesicles burst nicely under the UV, sparkling effects...

I also got a very informative answer from Lew Jacobson, which I
somehow have lost.  I remember two things from it.  First, he says
"The gut granules are definitely lysosomes", and cites this reference
to prove it:

    Clokey and Jacobson (1986) The autofluorescent lipofuscin granules
    in the intestinal cells of C. elegans are secondary
    lysosomes. Mechanisms of Ageing and Development 35: 79-84.

Second, he says daf-4 mutants have greatly reduced gut fluorescence
because they have greatly reduced endocytosis.

Answers came from the following:

Cori Bargmann		(cori@itsa.ucsf.edu)
Marty Chalfie		(chalfie@cubsps.bio.columbia.edu)
David Chitwood		(dchitwood@asrr.arsusda.gov)
Gaetan Borgonie		(Gaetan=Borgonie%reszoo%Biomse.RUG@vinarc01.rug.ac.be)
Michael Hengartner	(hengartn@cshl.org)
Andras Fodor		(fodoran@ludens.elte.hu)
Thomas Burglin		(BURGLIN@Frodo.MGH.Harvard.EDU)
Lewis Jacobson		(ljac@vms.cis.pitt.edu)

--
Leon Avery					   (214) 648-2420 (office)
Department of Biochemistry			            -2768 (lab)
University of Texas Southwestern Medical Center             -8856 (fax)
5323 Harry Hines Blvd				   leon@eatworms.swmed.edu
Dallas, TX  75235-9038

.