From owner-drosophila@net.bio.net Tue Aug 01 23:00:00 1995 Path: biosci!bcm!news.msfc.nasa.gov!newsfeed.internetmci.com!uunet!in2.uu.net!dziuxsolim.rutgers.edu!igor.rutgers.edu!newsserver.jvnc.net!paperboy.uconn.edu!UConnVM.UConn.Edu!AMB93002 From: AMB93002@UConnVM.UConn.Edu (Anja Baldo) Newsgroups: bionet.drosophila Subject: Food Media in Small Quantities? Date: Wed, 02 Aug 95 11:13:59 EDT Organization: University of Connecticut Lines: 27 Message-ID: <173EE9DF7S86.AMB93002@UConnVM.UConn.Edu> NNTP-Posting-Host: vm.ucc.uconn.edu Keywords: Food, Media Hi. Due to renovations, our usual fly kitchen is unavailable for making large batches of media. Instead, it appears we will need to make smaller batches on a rangetop in the interim. None of the recipes in appendix N of the grey book seem to have the same ingredients. My questions are these: 1) Has anyone any advice about reducing recipes? (Does it work, or are there adjustments I will need to make?) 2) Does anyone have a recipe on file for small batches (~2-4 L) of food with the following ingredients: (percentages are from the large recipe) 10.0% sucrose 6.0% yellow cornmeal 3.4% brewer's yeast 1.0% agar 1.6 vol Tegosept in EtOH I would like to be sure this food is *right*, since I am culturing other species than melanogaster, and they tend to prefer fresh food, made well. If this information is available online somewhere in flybase, I apologize for bothering you. I looked and didn't see it anywhere. Thanks very much. anja .