From owner-biotechniques@net.bio.net Wed Aug 13 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Anne Siri Langelandsvik Newsgroups: bionet.journals.letters.biotechniques Subject: Automated fluorescent primer extension Date: 14 Aug 1997 05:39:19 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <9708140756.AA25351@nlh10.nlh.no> NNTP-Posting-Host: net.bio.net Hi, I am going to do an automated fluorescent primer extension, that is using fluorescent labeled primer for the primer extension so that I can detect the band using an automatic DNA sequencer. However,do I need to use fluor. labeled primer for the DNA sequencing aswell,or can I do the "one" tube sequencing reaction involving fluor. labeled ddNTP's? The reason for this question is that I wonder if the extension product versus the sequencing products will run different because the primer is labeled with a "big" fluorochrom.=20 Anne Siri ................................................................... Anne Siri Langelandsvik Laboratory of Microbial Gene Technology Agricultural University of Norway P.O. Box 5051, N-1432 =C5s Tel: +47 64 94 85 96/94 69 Fax:: +47 64 94 14 65 Homepage: http://wwwnlh.nlh.no/institutt/ibf/ansatte/AL/ibf_ala.htm Private: S=F8r=E5sveien 18, N-1430 =C5s Tel: +47 64 94 45 16 ................................................................... .