From owner-drosophila@net.bio.net Thu May 02 23:00:00 1996
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From: Jonathan Kelty <Keltyj@miamiu.muohio.edu>
Newsgroups: bionet.cellbiol,bionet.drosophila
Subject: problems getting dipteran hemocytes to adhere
Date: 2 May 1996 19:46:42 GMT
Organization: Miami University
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Can anyone recommend a good protocol for adhering dipteran hemocytes to 
microscope slides?  

I am trying to find a protocol which will keep the cells attached to 
slides as they undergo a number of washes and incubations in preparation 
for viewing with immunofluorescence microscopy.  So far I have tried 
coating my slides with Poly-L-Lysine, Biobond and 3-aminopropyl-
triethoxysilane (APTES). Of these agents, APTES produced the greatest 
cell retention although I still lost most of the original cells.

Any advice will be greatly appreciated.

Jonathan Kelty (keltyj@miamiu.muohio.edu)
Department of Zoology
Miami University



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