From owner-biophysics@net.bio.net Wed Apr 01 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!Cabal.CESspool!bofh.vszbr.cz!lyra.csx.cam.ac.uk!not-for-mail From: Yu Wai Chen Newsgroups: bionet.molbio.methds-reagnts,bionet.biophysics Subject: assay for free cysteines Date: Thu, 02 Apr 1998 13:02:10 +0100 Organization: MRC Centre for Protein Engineering Lines: 14 Message-ID: <35237E42.4625143F@mrc-lmb.cam.ac.uk> NNTP-Posting-Host: i10.mrc-lmb.cam.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.04 [en] (X11; I; IRIX 6.2 IP22) Xref: biosci bionet.molbio.methds-reagnts:66263 bionet.biophysics:4103 Dear all, I would like to know if the 6 cysteines of my protein is disulphide-bridged or not. Can some one point me to a good reference or protocol how to achieve this? I've heard that we can use Ellman's reagent to do it... Is Ellman's reagent good for buried cysteines as well? Thanks. -- =================================================================== Yu Wai CHEN, Ph.D. .................. email: ywc@mrc-lmb.cam.ac.uk Centre for Protein Engineering, tel: 44-(1223) 402148 MRC Centre, Cambridge CB2 2QH, U.K. fax: 44-(1223) 402140 WWW homepage: http://www.mrc-cpe.cam.ac.uk/people/wai.html .