From owner-biophysics@net.bio.net Sun Feb 01 22:00:00 1998 Newsgroups: bionet.biophysics Path: biosci!agate!logbridge.uoregon.edu!ais.net!vixen.cso.uiuc.edu!uchinews!midway.uchicago.edu!aekentsi From: aekentsi@midway.uchicago.edu (alex emil kentsis) Subject: Re: stray light X-Nntp-Posting-Host: howard-nfs.uchicago.edu Message-ID: Sender: news@midway.uchicago.edu (News Administrator) Organization: The University of Chicago X-Newsreader: TIN [version 1.2 PL2] References: <34D2370B.31A7@york.ac.uk> Date: Mon, 2 Feb 1998 14:18:59 GMT Lines: 17 i am not sure if you are asking about background light levels, as from an ambient light leak, or about this strange phenomenon we've encountered. if the solution level in teh observation cuvette is below a certain level, the fluorimeter produces curved baselines. we think it's due to teh elevation of the meniscus in the cuvette which somehow produces stray light. if the meniscus is covered with a piece of tape or the solution volume increased so that it's far away from teh incident beam, the anomaly disappears. very strange. David Scott (djs17@york.ac.uk) wrote: : Hia : Does anyone out there know anything about stray light in a fluorimeter : and how to calculate/correct/get rid of it? : We have an application where this may be a problem : Thanks in advance : Dave Scott .