From owner-diagnost@hgmp.mrc.ac.uk Mon Jul 5 16:45:43 2004 Return-Path: X-Original-To: diagnost-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 5CD667D123 for ; Mon, 5 Jul 2004 16:45:43 +0100 (BST) X-Original-To: diagnost-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id DBB8C7D0FF for ; Mon, 5 Jul 2004 16:45:42 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6045) id A726D7D123; Mon, 5 Jul 2004 16:45:35 +0100 (BST) X-Original-To: bionet-diagnostics@net.bio.net Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 411CA7D34E; Wed, 26 May 2004 15:15:18 +0100 (BST) Received: from dexter.wzw.tum.de (dexter.wzw.tum.de [141.40.103.101]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 6BC6C7D347; Wed, 26 May 2004 15:15:10 +0100 (BST) Received: from pfaffl.wzw.tum.de ([141.40.217.71]) by dexter.wzw.tum.de (8.12.6/8.12.6/SuSE Linux 0.6) with ESMTP id i4QEF9dL000257; Wed, 26 May 2004 16:15:09 +0200 Message-Id: <5.2.1.1.0.20040526161642.028f7e70@pollux.wzw.tum.de> X-Sender: genequan@pollux.wzw.tum.de X-Mailer: QUALCOMM Windows Eudora Version 5.2.1 To: bio-srs@net.bio.net, bionauts@net.bio.net, cellbiol@net.bio.net, immuno@net.bio.net, molmodel@net.bio.net, microbio@net.bio.net, fluorpro@net.bio.net, bionet-diagnostics@net.bio.net, btechniq@net.bio.net, bionews@net.bio.net, bio-www@net.bio.net, 7tms_r@net.bio.net, zbrafish@net.bio.net, x-plor@net.bio.net, recom@net.bio.net, prenatal@net.bio.net, plant-ed@net.bio.net, phbiotec@net.bio.net, mol-evol@net.bio.net From: "qPCR.Gene-Quantification.Info Newsletter" Subject: May 2004 Mime-Version: 1.0 Content-Type: multipart/alternative; boundary="=====================_26938062==.ALT" Newsgroups: bionet.diagnostics Date: Mon, 5 Jul 2004 16:45:35 +0100 (BST) Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk --=====================_26938062==.ALT Content-Type: text/plain; charset="iso-8859-1"; format=flowed Content-Transfer-Encoding: quoted-printable Gene Quantification Newsletter May 2004 A new URL is defined for the Gene Quantification home page http://www.gene-quantification.info A lot of NEWS are presented: On HKG and normalization page: Guideline to reference gene selection for quantitative real-time PCR Selection of appropriate control genes to assess expression of tumor=20 antigens using real-time RT-PCR Comparison of RiboGreen=AE and 18S rRNA quantitation for normalizing=20 real-time RT-PCR expression analysis Novel Internal Controls For Real-Time PCR Assays Selection of optimal internal controls for gene expression profiling of=20 liver disease On real-time PCR page: Real-time PCR in the microbiology laboratory Quantitative RT-PCR: pitfalls and potential Real-time RT-PCR: Neue Ans=E4tze zur exakten mRNA Quantifizierung On chemistry page: http://dyes.gene-quantification.info/ Short summary of detection formats SYBR Green I BEBO TaqMan Probes LightCycler Hybridisation Probes Molecular Beacons Scorpions LNA Primers Light-Up Probes Q Zyme Assay Amplifluor Quantitative PCR Detection System LUX (Light Upon eXtension) primers The iCycler iQ Detection System for multiplex real-time PCR Assays A novel real-time quantitative PCR method using attached universal template= =20 probe Determination of real-time PCR efficiency All necessary informations about qPCR efficiency estimation http://efficiency.gene-quantifi= cation.info BioInformatics in real-time PCR A lot of applications related to qPCR or real-time PCR STATISTICS AND GENE EXPRESSION ANALYSIS by TERRY SPEED http://bioinformatics.gene-= quantification.info Endocrinology, Physiology and Immunology Application of real-time PCR in molecular Physiology, molecular=20 Endocrinology and molecular Immunology http://physiology.gene-quantifi= cation.info The 1st international qPCR Symposium & Application Workshop Please look at the nice pictures of the Symposium http://qpcr2004.gene-quantificati= on.info and Workshop talks are FREE for download http://qpcr= 2004.gene-quantification.info/qpcr2004-workshop.html Please send the NEWSLETTER and the=20 http://www.gene-quantification.info=20 home page links to further scientists and friends who are interested in=20 real-time PCR, new quantification strategies, relative or absolute gene=20 quantification using kinetic PCR. best regards Michael Pfaffl ############################################# For further questions concerning real-time PCR and if you want to SUBSCRIBE= =20 to our monthly appearing GENE QUANTIFICATION NEWSLETTER please contact=20 gene.quantification@wzw.tum.de You= =20 will receive all new features and future REST & REST-XL & BestKeeper=20 updates. GENE QUANTIFICATION web page and the GENE QUANTIFICATION NEWSLETTER are=20 educational sites with the only purpose of facilitating access to=20 biology-related information over the internet. The web page is optimised=20 for Netscape Navigator version 4.7 & Internet Explorer version 5.0 (and=20 higher versions) with an optimal screen resolution of 1024 x 768 pixels. Disclaimer & copyright on=20 http://www.gene-quantification.info=20 homepage. ******************************************************************** www.Gene-Quantification.Info mail: gene.quantification@wzw.tum.de homepage: http://www.gene-quantification.info PD Dr. Michael W. Pfaffl Physiology - Weihenstephan Life Science Center Weihenstephan, Technical University of Munich Weihenstephaner Berg 3 D-85350 Freising-Weihenstephan, GERMANY ******************************************************************** =20 --=====================_26938062==.ALT Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable

Gene Quantification Newsletter May 2004

A new URL is defined for the Gene Quantification home page

http://www.gene-quantificatio= n.info
 

A lot of NEWS are presented:

On HKG and normalization page:
Guideline to reference gene selection for quantitative real-time PCR
Selection of appropriate control genes to assess expression of tumor antigens using real-time RT-PCR
Comparison of RiboGreen=AE and 18S rRNA quantitation for normalizing real-time RT-PCR expression analysis
Novel Internal Controls For Real-Time PCR Assays
Selection of optimal internal controls for gene expression profiling of liver disease

On real-time PCR page:
Real-time PCR in the microbiology laboratory
Quantitative RT-PCR: pitfalls and potential
Real-time RT-PCR: Neue Ans=E4tze zur exakten mRNA Quantifizierung

On chemistry page:
http://dyes.gene-quantificat= ion.info/

Short summary of detection formats
SYBR Green I
BEBO
TaqMan Probes
LightCycler Hybridisation Probes
Molecular Beacons
Scorpions
LNA Primers
Light-Up Probes
Q Zyme Assay
Amplifluor Quantitative PCR Detection System
LUX (Light Upon eXtension) primers
The iCycler iQ Detection System for multiplex real-time PCR Assays
A novel real-time quantitative PCR method using attached universal template probe


Determination of real-time PCR efficiency

All necessary informations about qPCR efficiency estimation
http://efficiency.gene= -quantification.info

BioInformatics in real-time PCR

A lot of applications related to qPCR or real-time PCR
STATISTICS AND GENE EXPRESSION ANALYSIS by TERRY SPEED
http://bioinformat= ics.gene-quantification.info


Endocrinology, Physiology and Immunology

Application of real-time PCR in molecular Physiology, molecular Endocrinology and molecular Immunology
http://physiology.gene= -quantification.info


The 1st international qPCR Symposium & Application Workshop

Please look at the nice pictures of the Symposium
http://qpcr2004.gene-qua= ntification.info
and Workshop talks are FREE for download
ht= tp://qpcr2004.gene-quantification.info/qpcr2004-workshop.html

 
Please send the NEWSLETTER and the http://www.gene-quantificat= ion.info home page links to further scientists and friends who are interested in= real-time PCR, new quantification strategies, relative or absolute gene= quantification using kinetic PCR.

best regards

Michael Pfaffl

#############################################

For further questions concerning real-time PCR and if= you want to SUBSCRIBE to our monthly appearing GENE QUANTIFICATION= NEWSLETTER please contact gene.quantification@wzw.tum.= de   You will receive all new features and future REST= &  REST-XL  &  BestKeeper updates.

GENE QUANTIFICATION web page and the GENE QUANTIFICATION NEWSLETTER are= educational sites with the only purpose of facilitating access to= biology-related information over the internet. The web page is optimised= for Netscape Navigator version 4.7 & Internet Explorer version 5.0 (and= higher versions) with an optimal screen resolution of 1024 x 768= pixels.
 
Disclaimer & copyright on http://www.gene-quantificatio= n.info homepage.

******************************************************************= **
www.Gene-Quantification.Info

mail:       = ;    gene.quantification@wzw.tum.de
homepage:       http://www.gene-quantification.info

PD Dr. Michael W. Pfaffl
Physiology - Weihenstephan
Life Science Center Weihenstephan,
Technical University of Munich
Weihenstephaner Berg 3
D-85350 Freising-Weihenstephan, GERMANY
******************************************************************= ** --=====================_26938062==.ALT-- .